ROSA WET-S5 DON quantitative
No FGIS instruction found in the public library.
| Analyte | Deoxynivalenol |
| Toxin | Deoxynivalenol (DON) |
| Range | 0.5 – 5.4 / 4 – 30 mg/kg |
| Time | 5 min |
| Extraction | WET powder + water |
| Approval | Charm-validated (barley, maize, wheat) |
| Conformance | 0.5 – 30 mg/kg |
| Method | WET |
| Document | OM-648 · MRK-1044 |
From sample to result
Six steps. The first three determine the outcome more than the strip itself, and they are the most difficult to correct afterwards.
- Sampling: 50 g of ground material, at least 90 % passing through a 20-mesh sieve. Record lot, silo and date before you start.
- Extraction: WET: one pouch of powder plus water according to the manual, shake for 1.5 minutes and allow to stand for 10 minutes. Methanol: 70 % methanol according to the manual.
- Dilute: First dilution for the normal range; the second only for levels above the midpoint of the range.
- Application: 100 µl extract onto the strip, strip fully inserted into the slot of the EZ-M. One strip per test channel.
- Incubate and read: 45 ± 1 °C, duration according to the test. The instrument reads automatically and checks volume, reuse, lens and door.
- Result: Quantitative value in µg/kg or mg/kg, with test ID, sample ID and operator. EZ Receiver compares it against the maximum level for the product.
Controls and storage
- Test and record negative and positive controls weekly; EZ Receiver tracks them with custom limits and a trend line.
- Store strips at 18–30 °C; discard remaining strips after 12 hours.
- Compare the incubator temperature display daily against 45 °C.
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