ROSA FAST5 Zearalenone quantitative

Higher ranges apply to maize: 400 – 1 500 and 800 – 3 000 µg/kg.

Analyte	Zearalenone
Toxin	Zearalenone
Range	50 – 350 / 300 – 1 000 µg/kg
Time	5 min
Extraction	70% methanol
Approval	Charm-validated
Conformance	50 – 1 000 µg/kg
Method	solvent
Document	MRK-965 · brochure 2025

From sample to result

Six steps. The first three determine the outcome more than the strip itself, and they are the most difficult to correct afterwards.

1. Sampling: 50 g of ground material, at least 90 % passing through a 20-mesh sieve. Record lot, silo and date before you start.
2. Extraction: WET: one pouch of powder plus water according to the manual, shake for 1.5 minutes and allow to stand for 10 minutes. Methanol: 70 % methanol according to the manual.
3. Dilute: First dilution for the normal range; the second only for levels above the midpoint of the range.
4. Application: 100 µl extract onto the strip, strip fully inserted into the slot of the EZ-M. One strip per test channel.
5. Incubate and read: 45 ± 1 °C, duration according to the test. The instrument reads automatically and checks volume, reuse, lens and door.
6. Result: Quantitative value in µg/kg or mg/kg, with test ID, sample ID and operator. EZ Receiver compares it against the maximum level for the product.

Controls and storage

• Test and record negative and positive controls weekly; EZ Receiver tracks them with custom limits and a trend line.
• Store strips at 18–30 °C; discard remaining strips after 12 hours.
• Compare the incubator temperature display daily against 45 °C.

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